At Nautilus, we have a big and important mission: improve the health of millions by unleashing the potential of the proteome to accelerate drug development and enable a new world of precision and personalized medicine. We are developing a single-molecule protein analysis platform of unprecedented sensitivity, scale, and ease of use that we believe will democratize access to the proteome – one of the most dynamic and valuable sources of biological insight. To accomplish this, we are pursuing deep, hard science with an entrepreneurial mindset and creating a world-class team of builders, innovators, and dreamers across a wide range of disciplines.
We are looking for a skilled and motivated Senior Research Associate to join our affinity reagent discovery team. In this role, you will be a hands-on contributor to discovery campaigns including affinity reagent selection and characterization — from library panning through functional validation. You will provide both technical depth and the flexibility in a fast-moving, multi-project environment.
Responsibilities
Execute and optimize phage display panning campaigns to identify and enrich antigen-specific affinity reagent clones
Perform high-throughput screening and characterization using a variety of techniques, including ELISA, western blot, biolayer interferometry (BLI) and the Nautilus proteomics platform
Carry out molecular cloning workflows supporting antibody reformatting, expression construct design, and recombinant protein production
Culture mammalian cells (e.g., HEK293) for transient and stable protein expression
Contribute to data recording, experiment tracking, and reagent management within a LIMS platform
Present experimental results clearly and concisely to cross-functional teams
Author and maintain SOPs, assay protocols, and other controlled documentation
Take on dynamic task assignments across projects as team priorities evolve — a genuine willingness to wear multiple hats is essential
Requirements
Bachelor's degree or higher in Biochemistry, Molecular Biology, Cell Biology, or a related life science field
4–6 years of hands-on laboratory experience, with demonstrated proficiency in antibody-based assay development and reagent characterization
Direct experience with phage display or other affinity selection platforms
Proficiency in ELISA, western blot, and at least one biophysical binding method (BLI or SPR)
Practical experience with molecular cloning and mammalian cell culture
Experience working within a LIMS or equivalent data management system
Strong organizational skills and attention to detail, with the ability to manage multiple concurrent experiments
Clear written and verbal communication skills; comfortable presenting data to diverse audiences
Preferred Skills
Experience with fluorescence microscopy and/or cell-based imaging assays
Experience with automated liquid handling and magnetic bead-based workflows
Prior experience in a fast-paced biotech or startup research setting
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