Senior Scientist

The purpose of this position is to provide support in developing robust B cell screening platform within GBT leveraging GBT’s Biotherapeutic expertise for the Vaccine group to isolate pathogen specific monoclonal antibodies directly from donor individuals. By mining convalescent donor B cell repertoire using single cell culture and screening, we will learn how native humoral responses interact with pathogen. The platform is in early development and required further optimization. The incumbent, under the supervision by Pfizer Scientist, will be involved in the development and optimization of the platform and will develop appropriate assays in further increasing efficiency in identifying specific monoclonal antibodies against various pathogens 

• Develop and optimize single B cell culture condition and screening protocol for pathogen specific antigens 

• Develop and optimize single B cell IgG cloning condition 

• Express and purify antigen vaccine antigen specific monoclonal antibodies 

• Develop assays to further confirm functionality of isolated monoclonal antibodies